Western blot detection of infectious bursal disease virus infection

Western blot detection of infectious bursal disease virus infection.

In order to evaluate the use of a Western blot methodology for the diagnosis of infectious bursal disease virus (IBDV) infection, chickens were experimentally infected with IBDV strains and tested for the presence of viral antigens and antibodies by a blocking Western blot test (bWB). The viral proteins obtained from the bursa of Fabricius (BF) were transferred to a nitrocellulose membrane afte...

Characterization of a Highly Virulent Infectious Bursal Disease Virus

Comparative Analysis of Protein Profile of Vaccine Strain and Local Isolates of Avian Infectious Bursal Disease Virus by Western Blot

Infectious bursal disease (IBD), commonly known as “Gumboro Disease is an infectious viral disease of chickens inducing severe immunosuppression. In Pakistan, imported vaccine is used to prevent the disease but there have been outbreaks even in vaccinated flocks. This indicates that there is variation among the locally prevalent viral strains and commercially available vaccine strain. In presen...

Comparison between Micro and Tube Virus Neutralization Tests for Detection of Serological Responses to Infectious Bursal Disease Virus Following Vaccination

Conformational analysis of Infectious bursal disease virus

Aim: This study was designed to develop peptide analogs of Infectious Bursal Disease (IBD) virus VP5 protein segment having cell penetrating ability to improve their interaction with cargo molecule (Nucleic acid) without affecting the backbone conformation. Materials and Methods: IBDV VP5 protein segment designated as RATH peptide were synthesized using solid phase peptide synthesis and their s...